TaqI RFLP Identified by probe VK17A (DXS294) at Xq26

TaqI RFLP at D21S137.

Chromosomal Localization: The 3 polymorphic bands were mapped to chromosome 22 using a somatic cell hybrid panel. The 2.1 kb constant band mapped to chromosome lq whereas all other constant bands mapped to chromosome 20. Whereas the active gene for hTOPl is located on chromosome 20ql 1.2—13.1, two pseudogenes are on chromosomes Iq23—24 and 22qll.2-13.1 (2). The sequences of hTOPl and the two ps...

A TaqI RFLP identified at the retinoblastoma locus on chromosome 13.

SOURCE/DESCRIPTION: Probe D95HS0.5 is a 0.6 kb fragment subcloned into Blue-scribe a pUCl9 derivative from a bacterio phage library isolated by a cDNA probe of the retinoblastoma gene 1. The fragment is released with the enzymes Hindlll and Sail. POLYMORPHISMS: TaqI identifies a 2 allele polymorphism with a band at 2.1 kb and 1.8 kb with a frequency of 0.97 and 0.03 respectively. There are no c...

TaqI RFLP polymorphism of the ovine complement component C4 gene.

Protocol: Reactions were performed as described (2) using 1 ng of DNA and 100 pmoles of each primer. DNA was amplified for 20 cycles using a Perkin Elmer Cetus DNA Thermal Cycler: denaturation, 94°C, 1 min; annealing, 60°C, 1 min; and extension, 70°C, 1.5 min. Ten /il of the reaction was analyzed directly on a 2.5 % NuSieve GTG agarose gel run in Tns-borate buffer. The amplified DNA fragments v...

A TaqI RFLP for the human arylsulfatase A gene.

RFLP identified by the anonymous DNA segment FZ IV E11 at 1p23 [HGM9 no.D1S87].